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gγ 8 gfp2  (Addgene inc)


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    Structured Review

    Addgene inc gγ 8 gfp2
    G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET <t>(GFP2/Rluc8)</t> values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
    Gγ 8 Gfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g%CE%B3+8+gfp2/pmc11761627-87-47-50?v=Addgene+inc
    Average 92 stars, based on 2 article reviews
    gγ 8 gfp2 - by Bioz Stars, 2026-07
    92/100 stars

    Images

    1) Product Images from "Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations"

    Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations

    Journal: Biomedicines

    doi: 10.3390/biomedicines13010046

    G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
    Figure Legend Snippet: G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Techniques Used: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison

    Most abundant expressed G-protein combination activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean of the WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
    Figure Legend Snippet: Most abundant expressed G-protein combination activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean of the WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Techniques Used: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison



    Similar Products

    gγ 8 gfp2  (Addgene inc)
    92
    Addgene inc gγ 8 gfp2
    G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET <t>(GFP2/Rluc8)</t> values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.
    Gγ 8 Gfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g%CE%B3+8+gfp2/pmc11761627-87-47-50?v=Addgene+inc
    Average 92 stars, based on 1 article reviews
    gγ 8 gfp2 - by Bioz Stars, 2026-07
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Journal: Biomedicines

    Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations

    doi: 10.3390/biomedicines13010046

    Figure Lengend Snippet: G-protein activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean of the maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Article Snippet: Gα i1 -RLuc8 (Addgene, #140973), Gα i2 -RLuc8 (Addgene, #140974), Gα i3 -RLuc8 (Addgene, #140975), Gα oA -RLuc8 (Addgene, #140976), Gα oB -RLuc8 (Addgene, #140977), Gβ 1 (Addgene, #140987), Gβ 2 (Addgene, #166772), Gβ 3 (Addgene, #140988), Gγ 3 -GFP2 (Addgene, #166775), Gγ 7 -GFP2 (Addgene, #166778), Gγ 8 -GFP2 (Addgene, #140990), and Gγ 9 -GFP2 (Addgene, #140991) were gifts from Bryan Roth (Addgene kit #1000000163).

    Techniques: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison

    Most abundant expressed G-protein combination activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean of the WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Journal: Biomedicines

    Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations

    doi: 10.3390/biomedicines13010046

    Figure Lengend Snippet: Most abundant expressed G-protein combination activation of the WT, I212F, and M345R. ( A ) Concentration–response curves for the WT, I212F, and M345R with Gα i/o , β-, and γ-subunits as indicated, following dopamine application. netBRET (GFP2/Rluc8) values are normalized to the mean of the max dopamine response of the WT same-day control and reported as a percentage of the WT same-day control (% of G-protein activation (WT E max )). ( B ) Basal activity for the WT, I212F, and M345R are plotted as percentages of the mean of the WT E max same-day control (Basal activity (% of WT Max)). ( C ) Comparison of pEC 50 values for the WT, I212F, and M345R. ( D ) WT, I212F, and M345R maximal efficacy expressed as a percentage of the mean maximum of the WT same-day control (E max (% of WT E max )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test, or Student’s t -test as given in , and indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; ns—not significant; nd—not determined. Data shown as mean ± SEM.

    Article Snippet: Gα i1 -RLuc8 (Addgene, #140973), Gα i2 -RLuc8 (Addgene, #140974), Gα i3 -RLuc8 (Addgene, #140975), Gα oA -RLuc8 (Addgene, #140976), Gα oB -RLuc8 (Addgene, #140977), Gβ 1 (Addgene, #140987), Gβ 2 (Addgene, #166772), Gβ 3 (Addgene, #140988), Gγ 3 -GFP2 (Addgene, #166775), Gγ 7 -GFP2 (Addgene, #166778), Gγ 8 -GFP2 (Addgene, #140990), and Gγ 9 -GFP2 (Addgene, #140991) were gifts from Bryan Roth (Addgene kit #1000000163).

    Techniques: Activation Assay, Concentration Assay, Control, Activity Assay, Comparison